Sensory transduction in vertebrate olfactory receptor neurons
speaker: Jürgen Reingruber (ENS, Paris)abstract: The activation of odorant receptors located in the cilia of vertebrate olfactory receptor neurons (ORNs) initiates a signal transduction current that is largely carried by Ca2+-activated Cl- channels (Anoctamin 2, Ano2). However, neither the functional role of the Cl- current that can carry several hundred pA is fully understood, nor is it clear why the depolarizing current is anionic and not cationic as in photoreceptors, for example. Here we provide a putative explanation for the latter by studying the impact of a transduction current that is carried either by Cl- or Na+ on the ion dynamics in the constrained and small space of olfactory cilia. To carry out the analysis we developed a spatio- temporal model that takes into account the coupled dynamics of Na+, Ca2+, K+ and Cl-. We validated the model using experimental data and performed simulations to compare the consequences of an odorant response based on either a Cl- or Na+ current. Our main ndings are: First, contrary to a Cl- current, a Na+ current induces a large increase in the ciliary Na+ concentration during the odorant response. Second, contrary to a Na+ current, a transduction pathway based on a Cl- current is robust and little a ected by external Cl- or Na+ concentration changes in the mucus. Third, a Na+ current can increase the ciliary Na+ concentration to a level where the Na+=Ca2+=K+ exchanger mode is reversed and odorant response termination is compromised. In reverse mode the exchanger transports Ca2+ into the cilia, which can sustain the Ca2+-activated current even in absence of odorant stimulation. Fourth, contrary to a Cl- current, a Na+ current increases osmotic concentration and pressure in cilia during the odorant response, which might compromise the mechanical stability of fragile cilia because of potential water in ux.
timetable:
Thu 4 Oct, 15:15 - 16:30, Aula Dini
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